Monitoring of Phenol in Wastewater Bioremediation by HPLC

Bioremediation emphasizes the detoxification and destruction of toxic substances by microorganisms. Wastewater obtained from an industrial concern was solvent extracted with methyl alcohol and dichloromethane and analysed by GC/MS. Besides phenol, a large variety of organic compounds were detected. Under controlled laboratory conditions, the wastewater was innoculated with a mixed culture of microorganisms specially selected for their abilities to degrade phenol. Samples were collected at regular intervals from the stirred tank bioreactor and analysed for phenol by reverse phase HPLC with a C18 column. Results shows that from an initial phenol concentration of 987 ppm, slightly more than 50% was destroyed within 163 hours. The dry weight of the microorganisms and the plate count (CFU/ml) shows a steady increase from 0.5238 gms to 0.5355 gms and from 1.1E+9 to 1.94E+13 respectively over the same period. This suggested that the phenol was consumed by the microorganisms as the sole carbon source.     

Challenges in quantifying biosphere-atmosphere exchange of nitrogen species

Recent research in nitrogen exchange with the atmosphere has separated research communities according to N form. The integrated perspective needed to quantify the net effect of N on greenhouse-gas balance is being addressed by the NitroEurope Integrated Project (NEU). Recent advances have depended on improved methodologies, while ongoing challenges include gas-aerosol interactions, organic nitrogen and N(2) fluxes. The NEU strategy applies a 3-tier Flux Network together with a Manipulation Network of global-change experiments, linked by common protocols to facilitate model application. Substantial progress has been made in modelling N fluxes, especially for N(2)O, NO and bi-directional NH(3) exchange. Landscape analysis represents an emerging challenge to address the spatial interactions between farms, fields, ecosystems, catchments and air dispersion/deposition. European up-scaling of N fluxes is highly uncertain and a key priority is for better data on agricultural practices. Finally, attention is needed to develop N flux verification procedures to assess compliance with international protocols.     

Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel

Contamination of aquatic ecosystems with heavy metals has been receiving increased worldwide attention due to their harmful e ects on human health and other organisms in the environment. Most of the studies dealing with toxic e ects of metals deal with single metal species, while the aquatic organisms are typically exposed to mixtures of metals. Hence, in order to provide data supporting the usefulness of freshwater fish as indicators of heavy metal pollution, it has been proposed in the present study to investigate the bioaccumulation and depuration of chromium in the selected organs of freshwater fingerlings Cirrhinus mrigala, individually and in binary solutions with nickel. The results show that the kidney is a target organ for chromium accumulation, which implies that it is also the “critical” organ for toxic symptoms. The results further show that accumulation of nickel in all the tissues of C. mrigala is higher than that of chromium. In addition, the metal accumulations of the binary mixtures of chromium and nickel are substantially higher than those of the individual metals, indicating synergistic interactions between the two metals. Theoretically the simplest explanation for an additive joint action of toxicants in a mixture is that they act in a qualitatively similar way. The observed data suggest that C. mrigala could be suitable monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

Bioaccumulation of lead and the influence of chelating agents in Catla catla fingerlings

Lead is a widespread element and one of the persistent and cumulative pollutants of the environment. The present study deals with the bioaccumulation of lead and the influence of chelating agents, meso 2,3-dimercaptosuccinic acid (DMSA), D-Penicillamine and CaNa₂EDTA in reducing the concentration of lead on the selected organs of Catla catla fingerlings for both acute and chronic exposures by using ICP-AES. It is inferred from the present findings that there was a correlation between environmental conditions and the heavy metal contents of the fish. The highest concentration of lead is found in kidney tissues and the lowest in muscle tissues. The accumulation pattern of lead in the selected organs of Catla catla is: kidney > liver > gill > brain > muscle. Also, it has been found that the treatment of chelating agents, DMSA, D-Penicillamine and CaNa₂EDTA reduces the concentration of lead significantly for both acute and chronic exposures. The results also show that DMSA is the most effective chelator of lead in reducing the body burden of C. catla fingerlings. The observed data further indicate that C. catla could be suitable for monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

Potential impacts of early twenty-first century changes in temperature and precipitation on rainfed annual crops in the Central Andes of Peru

Smallholder agriculture in the Central Andes of Peru is based to large extent on rainfed cropping systems, is exposed to climatic risks and is expected to respond sensitively to increasing temperatures and shifts in the precipitation regime. Here, we examine the potential implications of early twenty-first century climate change scenarios for the cultivation of potato, maize, wheat, barley and broad bean, five annual crops that account for 50 % of the cultivated area in the Department of Cusco and Apurímac and provide the dietary backbone for a large share of the local population. The scenarios disclose a regionally coherent increase in temperature of the order of 1 °C but overall only moderate changes in growing season precipitation by 2030. A simple crop model is used to assess the effects of these changes on crop phenology and development. The results show earlier harvest dates, shorter cropping seasons and, in a few cases, a slightly higher risk of planting failure in the near future. This suggests that a better understanding of changes in the precipitation regime at the onset of the cropping season is required to evaluate short-term needs and possibilities for adaptation. However, as the scenarios are highly uncertain, these conclusions should be verified.     

Risk management strategies to cope with climate change in grassland production: an illustrative case study for the Swiss plateau

In this paper, we assess climate change impacts on an intensively managed grassland system at the Swiss Plateau using the process-based grassland model PROGRASS. Taking the CO₂ fertilization into account, we find increasing yield levels (in the range of 10–24%) and sharp increases in production risks for an illustrative climate change scenario that suggests a marked increase in temperature and decrease in summer rainfall. Climate change–induced increases in the coefficients of variation of grassland yields are in the range of 21 and 50%. This finding underpins that additional risk management strategies are needed to cope with climate-change impacts on grassland production. The outputs from the grassland model are evaluated economically using certainty equivalents, i.e., accounting for mean quasi rents and production risks. To identify potential risk management strategies under current and future climatic conditions, we consider adjustments of production intensity and farm-level yield insurance. The impact of climate change on production intensities is found to be ambiguous: farmers’ will increase intensity under unconstrained production conditions, but will decrease production intensity in the presence of a cross-compliance scheme. Our results also show that the considered insurance scheme is a powerful tool to manage climate risks in grassland production under current and future conditions because it can reduce the coefficients of variation of quasi rents by up to 50%. However, we find that direct payments tend to reduce farmers’ incentives to use such insurance scheme.     

Effect of acute concentration of zinc on the biochemical contents of brain of Labeo rohita: an FT-IR study

Heavy metal discharges to aquatic environment are of great concern due to their toxicity and accumulative behavior. Zinc is an essential trace element required for different physiological functions and plays important role in cellular metabolism. However, it becomes toxic when elevated concentrations are introduced into the environment. The aim of this work is to analyze zinc induced biochemical changes in the brain tissues of Labeo rohita fingerlings using Fourier Transformation Infrared Spectroscopy. Several important features have been observed in the zinc intoxicated brain tissues, namely, altered membrane lipid, altered protein profile and decreased glycogen content, indicating an alteration in the lipid and protein profiles leading to modification in membrane composition. Further, it is observed that the acute exposure to zinc causes some alteration in protein profile with a decrease in α-helix and an increase in random coil structures.     

Regional assessment of climate change impacts on maize productivity and associated production risk in Switzerland

A simple model of yield was used along with climate scenarios to assess the impact of climate change on grain maize productivity and associated economic risk in Switzerland. In a first application, changes in the precipitation regime alone were shown to affect the distribution of yield considerably, with shifts not only in the mean but also in the standard deviation and the skewness. Production risk was found to respond more markedly to changes in the long-term mean than in the inter-annual variability of seasonal precipitation amounts. In a further application, yield projections were generated with respect to a full climate scenario, with the emission pathway as specified in the IPCC A2 scenario. Anticipation of the sowing date was found to reduce the negative impact of climate change on yield stability, but was not sufficient to ensure average productivity levels comparable to those observed at present. We argued that this was caused by the reduction in the duration of the growing season, which had a stronger impact than suggested by previous studies. Assuming no change in price relations, the results also revealed a strong increase in production risk with climate change, with more than a doubling in the probability of yield falling short of a critical threshold as compared to today’s situation.