Response of the Ascorbate-Peroxidase of Selenastrum capricornutum to Copper and Lead in Stormwaters

The green alga Selenastrum capricornutum expresses a uniqueascorbate peroxidase, that responds to copper and lead. Attemptswere made to test if this peroxidase could be used to monitor thelevels of copper and lead in natural waters. When S.capricornutum was exposed to a stormwater sample, the specificactivity of the peroxidase in the cell extract was commensuratewith the combined copper and lead contents in the sample. Theperoxidase responses were also correlated with the 96 hr biomasstoxicity assay of S. capricornutum. However, unlike thebiomass toxicity assay, the peroxidase activity was not affectedby the anions in the samples. The use of this peroxidase can beused as a marker for testing heavy metal toxicity in the water.     

生物硝化池污水中硝化细菌的快速定量研究

实验采用聚合酶链式反应（PCR）技术与最大几率数法（MPN）相结合的MPN－PCR法对生物硝化池污水中的硝化细菌进行快速定量。所用的一对PCR引物是在对硝化细菌的16SrRNA基因进行系统比较的基础上设计合成的，可以扩增出大小为388bp的DNA片段。以从生物硝化池污水中抽提的含硝化细菌DNA的混合DNA为模板，进行PCR扩增并确定合适的扩增条件。运用MPN－PCR法进行定量检测的整个过程可在几小时之内完成。     

应用蚕豆根尖微核技术检测淮河水质的研究

本文报道利用蚕豆很尖激核技术研究淮河水的污染情况及致灾变性。结果表明，多数月份该水能诱导蚕豆很尖做校率的明显增高，对综合评价水质提供生物学指标。     

含铅,无铅汽油车尾气排出物遗传毒性比较

利用台架试验模拟汽车行驶工况，收集了发动机使用无铅汽油，含铅汽油燃烧后的尾气颗粒物，对其中吸附的有机物进行了提取，并以Ａｍｅｓ试验，中国仓鼠肺细胞体外微核实验等方法，从基因，染色体水平检测了两种颗粒提取的遗传毒性。     

国产十种合成洗衣粉诱发华西大蟾蜍(Bufo bufo andrewsi)蝌蚪红细胞微核的研究

研究了国产10种洗衣粉对华西大蟾蜍(Bufo bufo andrewsi)蝌蚪红细胞微核的诱变,结果表明“活力28超浓缩无泡加香”洗衣粉,“TM”及“SD”洗衣粉(1.2,6.0及12.0ppm)诱发蝌蚪微核率增高。在我们实验条件下,另外7种合成洗衣粉中的5种在12.0ppm浓度组也引起微核率升高。     

兰州市交通要道颗粒物污染状况及其致突变活性的研究

采用现场监测，色－质联用分析以及小鼠骨髓微核试验方法，评价了兰州市交通要道颗粒物的污染状况，分析了颗粒提取物（PE）的有机组成及致突变活性。结果表明，兰州市总悬浮颗粒物（TSP）污染严重。颗粒提取物中含有近100种有机化合物，其中包括致癌性多环芳烃。微核试验显示PE具有致突变活性，对人群健康的影响不容忽视。     

Prenatal diagnosis of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in a family with a previous fatal case of sudden unexpected death in childhood

Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is a potentially fatal inherited disease with a carrier frequency of approximately 1:100 in most Caucasian populations. The disease is implicated in sudden unexpected death in childhood. A prevalent disease-causing point mutation (A985G) in the MCAD gene has been characterized, thus rendering diagnosis easy in the majority of cases. Since the clinical spectrum of MCAD deficiency ranges from death in the first days of life to an asymptomatic life, there are probably other genetic factors—in addition to MCAD mutations—involved in the expression of the disease. Thus, families who have experienced the death of a child from MCAD deficiency might have an increased risk of a seriously affected subsequent child. In such a family we have therefore performed a prenatal diagnosis on a chorionic villus sample by a highly specific and sensitive polymerase chain reaction (PCR) assay for the G985 mutation. The analysis was positive and resulted in abortion. We verified the diagnosis by direct analysis on blood spots and other tissue material from the aborted fetus and from family members.     

新疆南疆部分地面饮用水的致突变性研究

饮用水的致突变性和潜在致癌危害是环境科学研究的重要内容,新疆南疆地区,除少数城市居民饮用自来水外,绝大多数农垦、牧区均以当地的河水、泉水和涝坝水等地面水为主要的饮用水源。为了解这些饮用水的致突变和潜在致癌危害,我们特在枯水季节,从具有代表性的塔什库尔干县某地的泉水(下称泉水)、河水(下称河水)、疏勒县城的深井     

Detection of Escherichia coli in wastewater based on enzyme immunoassay

This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 10⁴ cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost. Translated from Environmental Science, 2005, 26(5): 128–131 [译自: 环境科学]     

室内常见气传真菌孢子的细胞毒性研究

分别以MTT比色法和细胞克隆形成率实验，观察了室内常见气传真菌孢子对中国仓鼠（CHL）肺上皮细胞的存活和增殖能力的影响，并通过检测细胞培养液中乳酸脱氢酶（LDH）活力，胞内Ca^2 ,K^ 含量，观察真菌孢子对细胞膜通透性的影响，结果表明，室内常见气传真菌孢子能显影响CHL细胞的活性，并可使细胞膜通透性发生改变，引起胞内LDH外渗，细胞内外离子发生交换，细胞内K^ 浓度降低，而细胞外的Ca^2 有内流的趋势。