Development and validation of LC-MS/MS method for the determination of Ochratoxin A and its metabolite Ochratoxin α in poultry tissues and eggs

The objective of this study was to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Ochratoxin A (OTA) and Ochratoxin α (OTα) in poultry tissues and eggs. The two toxins were extracted by a mixture of acetonitrile/water, purified with a reversed phase C18 solid phase extraction column (SPE) and determined by LC-MS/MS. The LC-MS/MS method performances were evaluated in terms of linearity in solvent and in matrix (ranged from 0.5 to 15.10 µg L^?1 for OTA and from 0.60 to 17.85 µg L^?1 for OTα), limit of detection (LOD), limit of quantitation (LOQ), specificity, accuracy and precision in repeatability conditions. Recovery experiments were performed by spiking poultry liver, kidney, muscle and eggs around 1 µg kg^?1 and 10 µg kg^?1. LODs were 0.27 and 0.26 µg kg^?1 while LOQs were fixed at 1.0 and 1.2 µg kg^?1 for OTA and OTα, respectively. Main recoveries for OTA ranged from 82 to 109% and for OTα ranged from 55 to 89%. The values of within-laboratory relative standard deviation (RSD_r) were equal to or below 20%. Considering the results obtained and that all analytical performance criteria were fulfilled, the new extraction and purification method developed for OTA and OTα determination in animal tissues and eggs was found appropriate for control laboratories and research activities designed to ensure food safety.     

An LC-MS/MS method for creatine and creatinine analysis in paraquat-intoxicated patients

Abstract

A sudden increase in serum creatinine after paraquat intoxication has been reported in several clinical studies. However, this dramatic change of creatinine may be possibly due to an interconversion of creatine-creatinine in relation to paraquat toxicity. In order to investigate the creatine-creatinine relationship, a liquid chromatography tandem mass spectrometry in combination with electrospray ionization was developed and validated for simultaneous determination of creatine and creatinine in the serum. The chromatographic separation was achieved on a Gemini® C₆-Phenyl column with a gradient elution consisting of 0.1% formic acid in ultrapure water and methanol as the mobile phase. The method yielded suitable levels of specificity and selectivity, and calibration curves of creatine and creatinine in serum were linear over the concentration range of 0.5–200?µg mL^?1. The limit of quantification of both compounds was 0.5?µg mL^?1, and the method was accurate within the recovery range of 96.23–102.75%, indicating the robustness of the method. The method was successfully applied to toxicological samples from paraquat-intoxicated patients, and the concentrations of creatine and creatinine were quantified. High creatine concentrations in serum samples were observed which may lead to high serum creatinine despite normal kidney function as creatine is converted to creatinine in proportion to its concentration.     

Physiologically based pharmacokinetic modeling of perfluoroalkyl substances in the human body

Currently, there are limited data on the levels of perfluoroalkyl substances other than perfluorooctane sulfonic acid and perfluorooctanoic acid in the human body. Most of this information has been extracted from biological monitoring of plasma while the occurrence of perfluoroalkyl substances in other human tissues is rarely studied. The objective of the present study was to develop a physiologically based pharmacokinetic model to assess the concentration of perfluoroalkyl substances in human tissues, based on an existing model previously validated for perfluorooctane sulfonic acid and perfluorooctanoic acid. Experimental data on concentrations of perfluoroalkyl substances in human tissues from individuals in Tarragona County (Catalonia, Spain) were used to estimate the values of some distribution and elimination parameters needed for the simulation. No significant correlations were found between these parameters and the chain lengths. The model was finally validated for five perfluoroalkyl substances.     

Development of a Computationally Efficient Full Human Body Finite Element Model

Introduction: A simplified and computationally efficient human body finite element model is presented. The model complements the Global Human Body Models Consortium (GHBMC) detailed 50th percentile occupant (M50-O) by providing kinematic and kinetic data with a significantly reduced run time using the same body habitus.

Methods: The simplified occupant model (M50-OS) was developed using the same source geometry as the M50-O. Though some meshed components were preserved, the total element count was reduced by remeshing, homogenizing, or in some cases omitting structures that are explicitly contained in the M50-O. Bones are included as rigid bodies, with the exception of the ribs, which are deformable but were remeshed to a coarser element density than the M50-O. Material models for all deformable components were drawn from the biomechanics literature. Kinematic joints were implemented at major articulations (shoulder, elbow, wrist, hip, knee, and ankle) with moment vs. angle relationships from the literature included for the knee and ankle. The brain of the detailed model was inserted within the skull of the simplified model, and kinematics and strain patterns are compared.

Results: The M50-OS model has 11 contacts and 354,000 elements; in contrast, the M50-O model has 447 contacts and 2.2 million elements. The model can be repositioned without requiring simulation. Thirteen validation and robustness simulations were completed. This included denuded rib compression at 7 discrete sites, 5 rigid body impacts, and one sled simulation. Denuded tests showed a good match to the experimental data of force vs. deflection slopes. The frontal rigid chest impact simulation produced a peak force and deflection within the corridor of 4.63 kN and 31.2%, respectively. Similar results vs. experimental data (peak forces of 5.19 and 8.71 kN) were found for an abdominal bar impact and lateral sled test, respectively. A lateral plate impact at 12 m/s exhibited a peak of roughly 20 kN (due to stiff foam used around the shoulder) but a more biofidelic response immediately afterward, plateauing at 9 kN at 12 ms. Results from a frontal sled simulation showed that reaction forces and kinematic trends matched experimental results well. The robustness test demonstrated that peak femur loads were nearly identical to the M50-O model. Use of the detailed model brain within the simplified model demonstrated a paradigm for using the M50-OS to leverage aspects of the M50-O. Strain patterns for the 2 models showed consistent patterns but greater strains in the detailed model, with deviations thought to be the result of slightly different kinematics between models. The M50-OS with the deformable skull and brain exhibited a run time 4.75 faster than the M50-O on the same hardware.

Conclusions: The simplified GHBMC model is intended to complement rather than replace the detailed M50-O model. It exhibited, on average, a 35-fold reduction in run time for a set of rigid impacts. The model can be used in a modular fashion with the M50-O and more broadly can be used as a platform for parametric studies or studies focused on specific body regions.     

Citizen science networks in natural history and the collective validation of biodiversity data

Biodiversity data are in increasing demand to inform policy and management. A substantial portion of these data is generated in citizen science networks. To ensure the quality of biodiversity data, standards and criteria for validation have been put in place. We used interviews and document analysis from the United Kingdom and The Netherlands to examine how data validation serves as a point of connection between the diverse people and practices in natural history citizen science networks. We found that rather than a unidirectional imposition of standards, validation was performed collectively. Specifically, it was enacted in ongoing circulations of biodiversity records between recorders and validators as they jointly negotiated the biodiversity that was observed and the validity of the records. These collective validation practices contributed to the citizen science character or natural history networks and tied these networks together. However, when biodiversity records were included in biodiversity‐information initiatives on different policy levels and scales, the circulation of records diminished. These initiatives took on a more extractive mode of data use. Validation ceased to be collective with important consequences for the natural history networks involved and citizen science more generally.     

Modelling forest management within a global vegetation model—Part 2: Model validation from a tree to a continental scale

The construction of a new forest management module (FMM) within the ORCHIDEE global vegetation model (GVM) allows a realistic simulation of biomass changes during the life cycle of a forest, which makes many biomass datasets suitable as validation data for the coupled ORCHIDEE-FM GVM. This study uses three datasets to validate ORCHIDEE-FM at different temporal and spatial scales: permanent monitoring plots, yield tables, and the French national inventory data. The last dataset has sufficient geospatial coverage to allow a novel type of validation: inventory plots can be used to produce continuous maps that can be compared to continuous simulations for regional trends in standing volumes and volume increments. ORCHIDEE-FM performs better than simple statistical models for stand-level variables, which include tree density, basal area, standing volume, average circumference and height, when management intensity and initial conditions are known: model efficiency is improved by an average of 0.11, and its average bias does not exceed 25%. The performance of the model is less satisfying for tree-level variables, including extreme circumferences, tree circumference distribution and competition indices, or when management and initial conditions are unknown. At the regional level, when climate forcing is accurate for precipitation, ORCHIDEE-FM is able to reproduce most productivity patterns in France, such as the local lows of needleleaves in the Parisian basin and of broadleaves in south-central France. The simulation of water stress effects on biomass in the Mediterranean region, however, remains problematic, as does the simulation of the wood increment for coniferous trees. These pitfalls pertain to the general ORCHIDEE model rather than to the FMM. Overall, with an average bias seldom exceeding 40%, the performance of ORCHIDEE-FM is deemed reliable to use it as a new modelling tool in the study of the effects of interactions between forest management and climate on biomass stocks of forests across a range of scales from plot to country.     

The environmental attitudes inventory: A valid and reliable measure to assess the structure of environmental attitudes

Environmental attitudes (EA), a crucial construct in environmental psychology, are a psychological tendency expressed by evaluating the natural environment with some degree of favour or disfavour. There are hundreds of EA measures available based on different conceptual and theoretical frameworks, and most researchers prefer to generate new measures rather than organize those already available. The present research provides a cumulative and theoretical approach to the measurement of EA, in which the multidimensional and hierarchical nature of EA is considered. Reported are findings from three studies on the development of a psychometrically sound, multidimensional inventory to assess EA cross-culturally, the Environmental Attitudes Inventory (EAI). The EAI has twelve specific scales that capture the main facets measured by previous research. The twelve factors were established through confirmatory factor analyses, and the EAI scales are shown to be unidimensional scales with high internal consistency, homogeneity and high test-retest reliability, and also to be largely free from social desirability.     

降雨影响噪声自动监测数据的有效性判定分析

选取北京市近5年夏半年（4—9月）夜间的降雨数据及相关噪声自动监测小时等效声级，对小时雨量与噪声自动监测数据进行数学统计分析，找出影响噪声监测数据的小时雨量值及不同声级受雨噪声影响的雨量限值，作为降雨对噪声自动监测小时等效声级的有效性判定条件。     

Measurements and Modelling of Air Pollution in a Street Canyon in Helsinki

A measuring campaign was conducted in the street canyon 'Runeberg street' in Helsinki in 1997. Hourly concentrations of carbon monoxide (CO), nitrogen oxides (NO_X), nitrogen dioxide (NO₂) and ozone (O₃) were measured at the street and roof levels, and the relevant hourly meteorological parameters were measured at the roof level. The hourly street level measurements and on-site electronic traffic counts were conducted during the whole year 1997, and roof level measurements were conducted during approximately two months, from 3 March to 30 April in 1997. The Operational Street Pollution Model (OSPM) was used to calculate the street concentrations and the results were compared with the measurements. The overall agreement between measured and predicted concentrations was good for CO and NO_x, but the model slightly overestimated the measured concentrations of NO₂. The database, which contains all measured and predicted data, is available for a further testing of other street canyon dispersion models.     

Users' views of hospital environmental quality: Validation of the Perceived Hospital Environment Quality Indicators (PHEQIs)

Environmental Quality Perception (EQP) is an important construct used to help to understand the relationship between people and the hospital environment. From a patient-centered care perspective, it is important that hospital design take into account the patients' (and other users') point of view. This paper presents the adaptation and validation of a measure of hospital EQP, the Perceived Hospital Environment Quality Indicators (PHEQIs; Fornara, Bonaiuto, & Bonnes, 2006), and seeks to confirm the factor structure of this construct in a different cultural context. Three scales, two focusing on physical environments and one evaluating the social environment, were completed by 562 users of four orthopedic units in Portuguese hospitals, two older and two recently built or renovated. To assess criterion validity, hospital physical environments were also objectively evaluated by two architects. Using a confirmatory factor analysis the three validation procedures produced acceptable fit indices in the final measurement models. Overall reliability values were satisfactory, as was the evidence for criterion validity. PHEQIs scales and factors correlated with global evaluation of the environment, supporting concurrent criterion validity; and predictive criterion validity was demonstrated given that users of older and newer hospitals differed significantly on the perception of quality of hospital EQP, and that high congruence between users' and experts' evaluations was found. Discriminant construct validity was supported, and some difficulties in showing convergent validity are discussed in terms of item formulation adequacy. Implications for research and practice are described.