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Regional Environmental Change - Louisiana faces extensive coastal land loss which threatens the livelihoods of marginalized populations. These groups have endured extreme disruptive events in the...  相似文献   
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A multi-disciplinary approach was used to evaluate the health of yellow perch (Perca flavescens) in the St. Lawrence River (Quebec, Canada), which is experiencing a severe population decline in the downstream portion of the river. Physiological parameters, liver alterations, trace metal concentrations, parasite prevalence and abundance, stable isotope composition, and the presence/absence of the viral hemorragic septicemia virus (VHSV) were evaluated in perch collected at six sites along the river: Lake St. François, Lake St. Louis (north and south), Beauregard Island, and Lake St. Pierre (north and south). Trace metal concentrations in surface water were higher in Lake St. Louis and downstream of a major urban wastewater treatment plant discharge, indicating that this effluent was a significant source of Cu, As, Ag, Zn, and Cd. Levels of Pb in surface water exceeded thresholds for the protection of aquatic life in Lake St. Louis and were negatively correlated with body condition index in this lake. In Lake St. Pierre, Cu, Ag, and Cd bioaccumulated significantly in perch liver and lower body condition index and greater liver damage were observed compared to upstream sites. Parasite analyses indicated a higher abundance of metacercariae of the trematodes Apophallus brevis and Diplostomum spp. in Lake St. Louis, and VHSV was not detected in the liver of yellow perch for all studied sites. Overall, results suggested that the global health of yellow perch from Lake St. Pierre is lower compared to upstream studied sites, which could contribute to the documented population collapse at this site.  相似文献   
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Environmental Science and Pollution Research - Multi-biological level assessments have become great tools to evaluate the health of aquatic ecosystems. Using this approach, a complementary study...  相似文献   
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Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0–98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV.  相似文献   
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Little is known about the level and content of exposure to fine particles (PM2.5) among persons who attend fireworks displays and those who live nearby. An evaluation of the levels of PM2.5 and their elemental content was carried out during the nine launches of the 2007 Montréal International Fireworks Competition. For each event, a prediction of the location of the firework plume was obtained from the Canadian Meteorological Centre (CMC) of the Meteorological Service of Canada. PM2.5 was measured continuously with a photometer (Sidepak?, TSI) within the predicted plume location (“predicted sites”), and integrated samples were collected using portable personal samplers. An additional sampler was located on a nearby roof (“fixed site”). The elemental composition of the collected PM2.5 samples from the “predicted sites” was determined using both a non-destructive energy dispersive ED-XRF method and an ICP-MS method with a near-total microwave-assisted acid digestion. The elemental composition of the “fixed site” samples was determined by the ICP-MS with the near-total digestion method. The highest PM2.5 levels reached nearly 10 000 μg m?3, roughly 1000 times background levels. Elements such as K, Cl, Al, Mg and Ti were markedly higher in plume-exposed filters. This study shows that 1) persons in the plume and in close proximity to the launch site may be exposed to extremely high levels of PM2.5 for the duration of the display and, 2) that the plume contains specific elements for which little is known of their acute cardio-respiratory toxicity.  相似文献   
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As bioenergy production expands and new bioenergy-based technologies emerge, there is a growing concern regarding the sustainability of their ecological and socioeconomic impacts. Comprehensive sustainability assessments are needed to address this concern and to assure that the development of the bioenergy industry meets sustainability goals. Most sustainability assessments for bioenergy are expert-driven, broad, and largely motivated by an interest in optimizing international trade in bioenergy. As a result, social and cultural sustainability targets are vague or underdeveloped. In this study, we developed a sustainability assessment for a regional bioenergy production industry in Upper Michigan, using stakeholder participation. Semi-structured interviews and focus group meetings were used to elicit participants’ concerns and opinion. These concerns were translated into sustainability Criteria and Indicators (C&Is), many of which could be supported with available science. Some of the C&Is identified by participants were unique to the region. Sustainability C&Is were broadly categorized into economic (C = 5; I = 22), environmental (C = 6; I = 12), social (C = 3; I = 9), policy and regulations (C = 3; I = 13), and institutional capacity (C = 4; I = 13). While participants could identify indicators for most of the criteria (many of which are also supported by existing literature), further research and validation will be necessary to identify measurable, practical, and bias-free indicators for all criteria.  相似文献   
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