Electrocatalytic debromination of BDE-47 at palladized graphene electrode

Graphene electrodes （Ti/Gr） were prepared by depositing Gr sheets on Ti substrate, followed by an annealing process for enhancing the adhesion strength. Electrochemical impedance spectroscopies and X-ray dif- fraction patterns displayed that the electrochemical behavior of Ti/Gr electrodes can be improved due to the generation of TiO2 layer at Ti-Gr interface during the annealing process. The palladized Gr electrodes （Ti/Gr/Pd） were prepared by electrochemical depositing Pd nanoparticles on Gr sheets. The debromination ability of Ti/Gr/Pd electrodes was investigated using BDE-47 as a target pollutant with various bias potentials. The results indicated that the BDE-47 degradation rates on Ti/Gr/Pd electrodes increased with the negative bias potentials from 0V to -0.5 V （vs. SCE）. Almost all of the BDE-47 was removed in the debromination reaction on the Ti/Gr/Pd electrode at - 0.5 V for 3 h, and the main product was diphenyl ethers, meaning it is promising to debrominate completely using the Ti/Gr/Pd electrode. Although the debromination rate was slightly slower at -0.3 V than that under -0.5 V, the current efficiency at -0.3 V was higher, because the electrical current acted mostly on BDE-47 rather than on water.     

Using crosslinked polyvinyl alcohol polymer membrane as a separator in the microbial fuel cell

Separator between anode and cathode is an essential part of the microbial fuel cell （MFC） and its property could significantly influence the system perfor- mance. In this study we used polyvinyl alcohol （PVA） polymer membrane crosslinked with sulfosuccinic acid （SSA） as a new separator for the MFC. The highest power density of 7594-4 mW-m-2 was obtained when MFC using the PVA membrane crosslinked with 15% of SSA due to its desirable proton conductivity （5.16 x 10-2 S.cml）. The power density significantly increased to 11064- 30 mW.m-2 with a separator-electrode-assembly config- uration, which was comparable with glass fiber （11704- 46 mW.m-2）. The coulombic efficiencies of the MFCs with crosslinked PVA membranes ranged from 36.3% to 45.7% at a fix external resistance of lO00f2. The crosslinked PVA membrane could be a promising alter- native to separator materials for constructing practical MFC system.     

Microbial fuel cell with three-dimensional electrodes for domestic wastewater treatment and electricity generation北大核心CSCD

A single chamber microbial fuel cell (MFC) with three-dimensional electrodes packed bed carbon felts was developed to treat domestic wastewater while simultaneously generating electricity. The influence of batch and continuous operation mode on treatment effectiveness and electricity production of the MFC was investigated to provide a reference for the application of the MFC. The MFC with a total working volume of 1 440 mL was operated in the fed-batch mode for 5 d repeatedly three times, and then shifted to the continuous mode. During the testing of the continuous mode, wastewater was continuously pumped into the anode compartment at a flow rate of approximately 0.2 mL/min, resulting in a hydraulic retention time of 5 d. During the batch test, the MFC obtained 91.1% chemical oxygen demand (COD) and 98.2% NH4 +-N removal, which accorded with the first criteria specified in the discharge standard of pollutants for municipal wastewater treatment plants in China (GB18918-2002). A maximum power density of 27.88 mW/m3 was achieved at a 51 Ω external resistor. During the continuous test, the COD removal efficiencies ranged from 83.2% to 97.4%. The concentration of NH4 +-N gradually decreased within 5 d and was then maintained below 9.45 mg/L, thus an enhanced removal performance of NH4 +-N was acquired. However, a low removal efficiency of total nitrogen was observed owing to the accumulation of NO3 --N in the effluent since day 11. Additionally, the MFC continually generated electricity with a maximum power density of 582.5 mW/m3 and average output voltage of 0.087 7 V during the stable period in the continuous operation mode. Moreover, 16S rRNA gene high-throughput sequencing showed that Thauera sp., Saprospiraceae-UN sp., and OPB56-UN sp. were identified as dominant populations. The results suggested that the organic matter associated with power generation was constantly utilized by the microorganisms in the reactor, which caused an excellent electricity generation performance during the continuous test. Therefore, the continuous operation mode could improve the low output voltage phenomenon in the MFC. Thauera sp., as a type of nitrate-reducing bacteria, was enriched in the autotrophic denitrifying microbial communities; therefore, bio-enrichment with denitrifying bacteria such as Thauera sp. could decrease the concentration of NO3 --N in the effluent during the continuous operation mode, which is expected to be an innovation for improvement of wastewater treatment. © 2018 Science Press. All rights reserved.     

Charge and separation characteristics of nanofiltration membrane embracing dissociated functional groups

The current work focused on the investigation of charge and separation characteristics of nanofiltration （NF） membrane embracing dissociated functional groups under different electrolyte solutions. The electro-kinetic method was carried out to assess the membrane volume charge density （X） with different salt concentrations ranging from 0.1 to 10 mol. m-3 and different electrolyte species, such as type 1-1, type 2-1 and type 3-1. The Donnan steric pore model-dielectric exclusion （DSPM- DE） model was employed to evaluate the separation characteristics of the NF membrane for wide range of electrolyte concentration （from 25.7 to 598.9mol·m^-3）. The results indicated that the dissociation of the hydro- philic functional groups and the specific adsorption contributed to charge formation on membrane surface. The former played a dominant role in type 1-1 and type 2-1 electrolytes at dilute aqueous solutions （0.1-0.5 mol · m^3）. However, for type 3-1 electrolyte, specific adsorp- tion should contribute to the charge effect to a large extent. Moreover, the correlation between the volume charge density and feed concentration was in accordance with Freundlich isotherm. Furthermore, it was found that the separation characteristic of NF membrane could be evaluated well by DSPM-DE model coupling with electro-kinetic method in a whole concentration range.     

Scaling up a novel denitrifying microbial fuel cell with an oxic-anoxic two stage biocathode

A scaled up microbial fuel cell （MFC） of a 50 L volume was set up with an oxic-anoxic two-stage biocathode and activated semicoke packed electrodes to achieve simultaneous power generation and nitrogen and organic matter removals. An average maximum power density of 43.1 W·m^-3 was obtained in batch operating mode. By adjusting the two extemal resistances, the denitrification in the A-MFC and power production in the O-MFC could be enhanced. In continuous mode, when the hydraulic retention times were set at 6 h, 8 h and 12 h, the removal efficiencies of COD, NHf-N and total nitrogen （TN） were higher than 95%, 97%, and 84%, respectively. Meanwhile the removal loads for COD, NH4^＋-N and TN were10, 0.37 and 0.4 kg·（m^3·d）^-1, respectively.     

Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions

This study compared the growth and lipid accumulation properties of two oleaginous microalgae, namely, Scenedesmus sp. LX1 and Chlorella sp. HQ, under different nutrient conditions. Both algal species obtained the highest biomass, lipid content and lipid yield under low-nutrient conditions （mBGll medium）. The biomass, lipid content and lipid yield of Scenedesmus sp. LX1 were 0.42g·L^-1, 22.5% and 93.8mg·L^-1, respectively. These values were relatively higher than those of Chlorella sp. HQ （0.30g·L^-1, 17.1% and 51.3mg·L^-1, respectively）. These algae were then cultivated in an SE medium that contained more nutrients; as a result, the biomass and lipid yield of Scenedesmus sp. LX1 reduced more significantly than those of Chlorella sp. HQ. Opposite results were observed in lipid and triacylglycerols （TAGs） contents. The cell sizes of both algal species under low-nutrient conditions were larger than those under high-nutrient conditions. Chlorella sp. HQ cells did not aggregate, but Scenedesmus sp. LX1 cells flocculated easily, particularly under low-nutrient conditions. In summary, low-nutrient conditions favour the growth and lipid production of both algae, but Scenedesmus sp. LX1 outperforms Chlorella sp. HQ.     

Effects of shear force on formation and properties of anoxic granular sludge in SBR

This paper reports the effects of shear force on anoxic granular sludge in sequencing batch reactors （SBR）. The study was carried out in two SBRs （SBR1 and SBR2） in which sodium acetate （200mg COD·L^-1） was used as the sole substrate and sodium nitrate （40 mgNO3-N·L^-1） was employed as the electron acceptor. The preliminary objective of this study was to cultivate anoxic granules in the SBR in order to investigate the effects of shear force on the formation of anoxic granular sludge and to compare the properties of anoxic sludge in the SBR. This study reports new results for the values of average velocity gradient, a measure of the applied shear force, which was varied in the two SBRs （3.79 s^-1 and 9.76 s^-1 for SBR1 and SBR2 respectively）. The important findings of this research highlight the dual effects of shear force on anoxic granules. A low shear force can produce large anoxic granules with high activity and poor settling ability, whereas higher shear forces produce smaller granules with better settling ability and lower activity. The results of this study show that the anoxic granulation is closely related to the strength of the shear force. For high shear force, this research demonstrated that： 1） granules with smaller diameters, high density and good settling ability were formed in the reactor, and 2） granular sludge formed faster than it did in the low shear force reactor （41days versus 76 days）. Once a steady-state has been achieved, the nitrate and COD removal rates were found to be 98% and 80%, respectively. For low shear force, such as was applied in SBR1, this research demonstrated that： 1） the activity of anoxic granular sludge in low shear force was higher than that in high shear force, 2） higher amount of soluble microbial products （SMPs） were produced, and 3） large pores were observed inside the larger granules,which are beneficial for nitrogen gas diffusion. Electron microscopic examination of the anoxic granules in both reactors showed that the morphology of the granules was ellipsoidal with a clear outline. Coccus and rod-shaped bacteria were wrapped by filamentous bacteria on the surface of granule.     

Electrochemical oxidation of humic acid at the antimony- and nickel-doped tin oxide electrode

This work investigated the degradation of humic acid （HA） in aqueous solution by electrochemical oxidation with Antimony- and Nickel-doped Tin oxide electrode （Ni-Sb-SnO2/Ti electrode） as the anode. Initial concentrations of HA ranged from 3 to 9 mg-L 1. Under such a concentration scope, the degradation of HA was a mass transfer controlled process. Degradation rate increased with the increase of HA initial concentration. Test on the effect of tert-butanol revealed that · OH played an important role in the oxidation of HA. The absence of cation Ca2＋ was beneficial to HA degradation, which suggested that both indirect and direct electrolyze happened during the whole electrochemical oxidation process. Alkaly （pH = 12） and neutral （pH = 7） conditions were benefical to HA degradation.     

Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

In this study, microorganisms （named B111） were immobilized on polyvinyl alcohol microspheres prepared by the inverse suspension crosslinked method. The biodegradation of bisphenol A （BPA） and 4-hydro- xybenzaldehyde, a degradation product of BPA, by free and immobilized B lll was investigated. The BPA degradation studies were carried out at initial BPA concentrations ranging from 25 to 150 mg·L^-1. The affinity constant Ks and maximum degradation rate Rmax were 98.3 mg·L^-1 and 19.7mg·mg^-1VSS·d^-1 for free B111, as well as 87.2mg·L^-1 and 21.1mg·mg^-1VSS·d^-1 for immobilized B 111, respectively. 16S rDNA gene sequence analyses confirmed that the dominant genera were Pseudomonas and Brevundimonas for BPA biodegradation in microorganisms B 111.     

Combined biologic aerated filter and sulfur/ceramisite autotrophic denitrification for advanced wastewater nitrogen removal at low temperatures

An innovative advanced wastewater treatment process combining biologic aerated filter （BAF） and sulfur/ ceramisite-based autotrophic denitrification （SCAD） for reliable removal of nitrogen was proposed in this paper. In SCAD reactor, ceramisite was used as filter and Ca （HCO3）2 was used for supplying alkalinity and carbon source. The BAF-SCAD was used to treat the secondary treatment effluent. The performance of this process was investigated, and the impact of temperature on nitrogen removal was studied. Results showed that the combined system was effective in nitrogen removal even at low temperatures （8℃）. Removal of total nitrogen （TN）, NH4＋ -N, NO3-N reached above 90% at room temperature. Nitrification was affected by the temperature and nitrification at low temperature （8℃） was a limiting factor for TN removal. However, denitrification was not impacted by the temperature and the removal of NO3 -N maintained 98% during the experimental period. The reason of effective denitrification at low temperature might be the use of easily dissolved Ca（HCO3）2 and high-flux ceramisite, which solved the problem of low mass transfer efficiency at low temperatures. Besides, vast surface area of sulfur with diameter of 2-6 mm enhanced the rate of microbial utilization. The removal of nitrate companied with the production of SO42-, and the average concentration of SO27 was about 240mg.L^-1. These findings would be beneficial for the application of this process to nitrogen removal especially in the winter and cold regions.     

Occurrence of odor problems in drinking water of major cities across China

A comprehensive investigation into the occur-rence of odor problem at 111 drinking water treatment plants （DWTPs） in major cities across China was undertaken using both flavor profile analysis （FPA） and gas chromatography-mass spectrometry （GC-MS）. Eighty percent of source water samples exhibited odor problems, characterized by earthy/musty （41%） and swampy/septic （36%） odors, while the occurrence rate was lower （45%） in the finished water. Source water from rivers exhibited more pollution-origin odors, such as the swampy/septic odor, while that from lakes and reservoirs exhibited more algae- origin odors, such as earthy/musty odors. The occurrence rate of 2-methylisoborneol （2-MIB） in the surface source water samples was 75%, with 7% of samples containing 2- MIB concentrations of over 10 ng.L-1. The earthy/musty odor in the lake/reservoir water samples was mainly caused by 2-MIB （linear regression coefficient, R2= 0.69）, while the correlation between 2-MIB concentration and the earthy/musty odor intensity samples was weak （R2= 0.35） in the river-source water These results will be useful for the management of odor-quality problems in drinking water of China.     

Adsorption of benzene and toluene from waste gas using activated carbon activated by ZnCl2

A series of activated carbons with high surface area were prepared from walnut shell using chemical activation with ZnCl2. In this research the carbonization stage was carried out at 500℃. The performance of the synthesized carbons evaluated in adsorption of benzene and toluene from waste gas. The influence of impregnation ratio on the characteristics of synthesized activated carbons as well as their adsorption capacity was investigated. The ratio of activation agent to walnut shell was selected in the range of 0.5-2.0 wt/wt. The synthesized activated carbons were characterized using XRD, SEM, BET and FTIR techniques. The highest activated carbon production yield was obtained at impregnation ratio of 1.5 wt/wt. The XRD analysis illustrated that peaks intensity decreased with increasing impregnation ratio showing that amorphous property of samples was increased. The SEM analysis revealed successful pore development in synthesized activated carbons obtained at high impregnation ratios. The surface area of the activated carbons increased with increasing impregnation ratio and its maximum value reached 2643 m2.g 1 at impregnation ratio of 2/1. FTIR analysis indicated that the relative amount of different acidic surface groups on synthesized carbons was a function of impregnation ratio. Experimental results for benzene and toluene adsorption showed a high potential of employing synthesized impregnated activated carbon for treatment of waste gas. Generally, the amount of VOC adsorbed on the surface was affected by physicochemical properties of synthesized activated carbons.     

A red water occurrence in drinking water distribution systems caused by changes in water source in Beijing,China: mechanism analysis and control measures

A red water phenomenon occurred in several communities few days after the change of water source in Beijing, China in 2008. In this study, the origin of this problem, the mechanism of iron release and various control measures were investigated. The results indicated that a significant increase in sulphate concentration as a result of the new water source was the cause of the red water phenomenon. The mechanism of iron release was found that the high-concentration sulphate in the new water source disrupted the stable shell of scale on the inner pipe and led to the release of iron compounds. Experiments showed that the iron release rate in the new source water within pipe section was over 11-fold higher than that occurring within the local source water. The recovery of tap water quality lasted several months despite ameliora- tive measures being implemented, including adding phosphate, reducing the overall proportion of the new water source, elevating the pH and alkalinity, and utilizing free chlorine as a disinfectant instead of chloramine. Adding phosphate was more effective and more practical than the other measures. The iron release rate was decreased after the addition of 1.5 mg. L-1 orthophosphate- P, tripolyphosphate-P and hexametaphosphate-P by 68%, 83% and 87%, respectively. Elevating the pH and alkalinity also reduced the iron release rate by 50%. However, the iron release rate did not decreased after replacing chloramine by 0.5-0.8 mg. L-1 of free chlorine as disinfectant.     

Characterization of phosphorus species and modeling for its organic forms in eutrophic shallow lake sediments,North China

Variations of phosphorus （P） and its species in surface sediment of Baiyangdian Lake, a eutrophic shallow lake located in North China, were investigated through combination of field survey and numerical calculation based on cluster analysis. P fractionation was performed by a sequential extraction scheme, categorized as loosely bound P （NH4Cl-P）, reductant soluble P （BD-P）, metallic oxide bound P （NaOH-P）, calcium bound P （HCl-P） and organic P （Org-P）. P concentrations exhibited regional similarities and a total of four sub-areas were identified in which the same rank was HCl-P 〉 Org-P 〉 BD-P ,=NaOH-P 〉NH4Cl-E NH4Cl-P, BD-P and Org-P were found to contribute to P enrichment in overlying water column. Specifically, labile Org-P acted as a potential pool with a greater contribution in aerobic layer compared to anaerobic layer. A hysteresis （lag = 4 months） existed when labile Org-P concentration was negatively correlated with aerobic layer thickness. In view of magnitude of identified P contributors in sub-areas, higher potential of P release was present in Fuhe River and Tang River estuary areas. On the basis of calibration and verification, the mathematical model with parameter settings applied in this study was improved to serve as a tool for limnology management and eutrophic control.     

Reuse of heavy metal-accumulating Cynondon dactylon in remediation of water contaminated by heavy metals

Phytoremediation technology is regarded as a simple and efficient way to remove heavy metals from contaminated soil. A reasonable disposal of metal hyperaccumulators is always and resource-saving. The a major issue in waste reuse heavy metal-accumulating Cynondon dactylon （L.） was investigated where heavy metals were desorbed by a facile acid-treatment. The result indicated that more than 90% of heavy metals （Zn, Pb and Cu） was extracted from Cynondon daetylon with 0.2 mmol· L^-1 HCl. The plant residue was used to adsorb heavy metals ions. The adsorption fitted the Langmuir isotherm model with the saturation adsorption capacity of 9.5 mg·g^-1 Zn^2＋, 36.2 mg·g^-1 Pb2＋ and 12.9 mg·g^-1 Cu^2＋, and the surface eomplexation and the backfilling of heavy metal imprinting cavities existed simultaneously during the adsorption. The treatment of wastewaters indicated that the plant residue exhibited a high removal rate of 97% for Cu. Also, the material could be recycled. The method offers a new disposal approach for heavy metal hyperaccumulator.     

Neighborhood form and CO2 emission: evidence from 23 neighborhoods in Jinan, China

To understand the household C02 emission level in China, as well as how much the neighborhoods＇ socio-economic or design factors could influence the CO2 emission, 23 neighborhoods in Jinan were investigated in 2009 and 2010. These neighborhoods fall into four different types： superblock, enclave, grid and traditional. The household CO2 emission includes sources of both in- home energy use and passenger transportation. The average CO2 emission per household is 7.66 t.a^-1, including 6.87 t in-home operational emission and 792 kg transportation emission. The household CO2 emission by neighborhood categories is 10.97, 5.65, 6.49, 5.40 t-household-1. a-1 for superblock, enclave, grid and tradi- tional respectively. Superblock has the highest average emission and also the highest percent （more than 25%） of transportation emission among four different types of neighborhoods. The residential CO2 emission of superb- lock neighborhoods in Jinan has already reached the level in developed countries nearly ten years ago. It is predictable that more superblock neighborhoods would be built in China with the fast urbanization. How to avoid the rapid household CO2 emission growth in the future would be a systematic issue. The study also found that in addition to income and apartment area, household density, land use mix and accessibility to public transportation are three primary factors which have significant impacts on CO2 emission. High density, mixed land use and convenient accessibility to public transportation tend to reduce household CO2 emission.     

Simultaneous quantification of several classes of antibiotics in water,sediments, and fish muscles by liquid chromatography-tandem mass spectrometry

Precise and sensitive methods for the simultaneous determination of different classes of antibiotics, including sulphonamides, fluoroquinolones, macrolides, tetracyclines, and trimethoprim in surface water, sediments, and fish muscles were developed. In water samples, drugs were extracted with solid-phase extraction （SPE） by passing 1000 mL of water through hydrophilic lipophilic balanced （HLB） SPE cartridges. Sediment samples were solvent-extracted, followed by tandem SPE （strong anion exchange （SAX） ＋ HLB） clean-ups. Fish muscles were extracted by a mixture of acetonitrile and citric buffer （80：20, v/v） solution, and cleaned by SPE. Liquid chromatography-tandem mass spectrometry （LC-MS/ MS） with multiple reaction monitoring （MRM） detection was employed to quantify all compounds. The recoveries for the antibiotics in the spiked water, sediment, and fish samples were 60.2%-95.8%, 48.1%-105.3%, and 59.8%- 103.4%, respectively. The methods were applied to samples taken from Dianchi Lake, China. It showed that concentrations of the detected antibiotics ranged from limits of quantification （LOQ） to 713.6 ng- L1 （ofloxacin） in surface water and from less than LOQ to 344.8 μg·kg-1 （sulphamethoxazole） in sediments. The number of detected antibiotics and the overall antibiotic concentrations were higher in the urban area than the rural area, indicating the probable role of livestock and human activities as important sources of antibiotic contamination. In fish muscles, the concentration of norfioxacin was the highest （up to 38.5 μg·kg-1）, but tetracyclines and macrolides were relatively low. Results showed that the methods were rapid and sensitive, and capable of determining several classes of antibiotics from each of the water, sediment, and fish matrices in a single run.     

混合菌群生物燃料电池的产电机理与特性

利用厌氧污泥为接种源构建双室微生物燃料电池(Microbial fuel cell,MFC),研究其电子传递机制,并考察其底物利用谱及阴极电子受体对产电性能的影响.结果表明:该MFC主要通过生物膜机制实现电子从有机物到固体电极的传递过程.该混合菌MFC的底物利用谱范围广泛,单糖、二糖、小分子有机酸等有机物均可作为电子供体产电,其中以蔗糖和乳糖为底物产电效果较好,最大功率密度分别为69.69 mW/m2和60.75 mW/m2;而以乙醇为底物时,COD负荷最高,达123.55 mg L-1d-1.阴极不同电子受体对混合菌群MFC的产电性能也有显著影响,其中以KMnO4为电子受体电池性能最好,最大功率密度达1 396.74 mW/m2.     

E. coli and bacteriophage MS2 disinfection by UV,ozone and the combined UV and ozone processes

The combination of low-dose ozone with ultraviolet （UV） irradiation should be an option to give benefit to disinfection and reduce drawbacks of UV and ozone disinfection. However, less is known about the disinfection performance of UV and ozone （UV/ozone） coexposure and sequential UV-followed-by-ozone （UV- ozone） and ozone-followed-by-UV （ozone-UV） expo- sures. In this study, inactivation of E. coli and bacterioph- age MS2 by UV, ozone, UV/ozone coexposure, and sequential UV-ozone and ozone-UV exposures was investigated and compared. Synergistic effects of 0.5-0.9 log kill on E. coli inactivation, including increases in the rate and efficiency, were observed after the UV/ozone coexposure at ozone concentrations as low as 0.05 mg-L-1 in ultrapure water. The coexposure with 0.02-mg.L-1 ozone did not enhance the inactivation but repressed E. coli photoreactivation. Little enhancement on E. coli inactivation was found after the sequential UV-ozone or ozone-UV exposures. The synergistic effect on MS2 inactivation was less significant after the UV/ozone coexposure, and more significant after the sequential ozone-UV and UV-ozone exposures, which was 0.2 log kill for the former and 0.8 log kill for the latter two processes, at ozone dose of 0.1 mg. t-1 and UV dose of 8.55 mJ. cm 2 in ultrapure water. The synergistic effects on disinfection were also observed in tap water. These results show that the combination of UV and low-dose ozone is a promising technology for securing microbiological quality of water.     

Cadmium and lead toxicity and bioaccumulation in Microcystis aeruginosa

The growth of human population leads to intensification of agriculture and promotes, through eutrophication, development of cyanobacteria. One of the most widespread and bloom-forming species in freshwater is toxic Microcystis aeruginosa （M. aeruginosa）. Combustion of fossil fuels and metallurgical processes are the main sources of heavy metals contamination in surface water including cadmium （Cd） and lead （Pb）. The following study was conducted in order to determine the effect of 1- 20 mg. L-1 of Cd and Pb on photochemistry （using flow cytometry） and growth （based on chlorophyll concentra- tion） ofM. aeruginosa as well as to estimate levels of metal bioaccumulation. We have found that 1-10mg.L-1 of Cd and 1-5 rag. L1 of Pb induced continuous enhancement of chlorophyll fluorescence during 24 h of incubation. No significant degradation of chlorophyll was observed in these samples. At higher concentrations of 20 mg. L-1 of Cd and 10-20 mg.L-1 of Pb chlorophyll level significantly decreased and its fluorescence was quenched. M. aeruginosa demonstrated high capability of Cd and Pb bioaccumulation, proportionally to initial metal concentration. In samples with initial concentration of 20 mg. L-1 of Cd and Pb bioaccumulation of 87.3% and 90.1% was observed, respectively. Our study demonstrates that M. aeruginosa can potentially survive in highly metals polluted environments, be a primary source of toxic metals in the food chain and consequently contribute to enhanced toxicity of heavy metals to living organisms including human.