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111.
Liver alterations were stereologically determined in five groups, each of 10 male and female Sprague–Dawley weanling rats fed PCB 105 (2,3,3′,4,4′-pentachlorobiphenyl) for 13 weeks at concentrations of 0.05, 0.5, 5, and 50?ppm mixed with corn oil. Animals from two groups receiving the oil in diets served as controls for each gender. A significant increase in mean volume fraction (VF) of smooth endoplasmic reticulum (SER) profiles was estimated in hepatocytes of female rats in 0.5, 5, and 50?ppm groups. A significant decrease in VF of mitochondria in female rats at 50?ppm was detected. In addition, a decrease in VF of lipid droplets was measured in female rats at 0.5, 5, and 50?ppm groups. No difference was found in VF of rough endoplasmic reticulum (RER) cisternae, and peroxisome profiles at any concentration of the PCB in either male or female animals as compared to baseline values from controls. A significant elevation of baseline VF for RER in female rats was detected in comparison with males. In the present study, no observable adverse effect level (NOAEL) for PCB 105 was estimated at <0.5?ppm for hepatocyte alterations in female Sprague–Dawley rats. 相似文献
112.
This study was carried out to determine changes in the blood and liver of rats given oral doses of cadmium (Cd) and zinc (Zn). Thirty 3 month old rats were kept six to a compartment in a well-demarcated five compartment cage labeled A, B, C, D, and E. After 2 weeks of acclimatization, and with ad libitum administration of water and feed, group A and B rats were, respectively, exposed to 100 mg CdSO4 and 2200 mg ZnSO4, respectively, mixed with their feed. Group C rats were fed with a combination of 100 mg CdSO4 and 2200 mg ZnSO4 mixed with their feed, while group D rats were exposed to 100 mg CdSO4 mixed with their feed for 48 h, and after they were given 2200 mg ZnSO4 for 96 h. Group E rats served as control and were fed a normal diet. Analysis of blood samples after 5 days showed that in rats of groups A, B, C, and D, WBC count increased from 6800 to 12,400 mm?3 and platelets from 26,000 to 88,000 mm?3. Reduction in blood counts were found for RBC from 13.8 to 4.7 × 106 mm?3, hemoglobin from 15.6 to 11.4 dL?1, and PCV from 47 to 34%. ESR was the same 1.0 mm h?1 in all groups. Severe damage to the liver was also shown by severe degeneration of hepatocytes and increase in Kuppfer cells. The above observations were mildest in the liver of group C rats. A reduction of body weight was observed in all treatment groups. Cd and Zn were found to be hematoxic and hepatotoxic in the Norwegian rat with Cd exhibiting greater toxicity than Zn. Toxicity of Cd was suppressed by Zn only when both metals were admistered at the same time, but a delay in intake of Zn (after 48 h) had little effect on Cd toxicity. 相似文献